Immunohematology Programs

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IMMUNOHEMATOLOGY, BASIC

Five paired, washed cells and serum per testing event for the following analytes:


ABO Group

D (Rho) Typing


ABO Group - D (Rho) Typing

  1. The paired cells and serum should be used for forward and reverse typing.
  2. Code all your answers using only the numbers available on the reporting form.
  3. Laboratories not performing sub-grouping of A should use code 443 in ABO Grouping.
  4. Laboratories not performing the weak D (Du) test on D (Rho) negative specimens must report code 451 for Negative, weak D (Du) not performed. Laboratories performing the weak D (Du) test on D (Rho) negative specimens must report either code 010 for negative or code 4 for weak D (Du) positive.
  5. If code 010 (negative) is reported, it is assumed the weak D test was performed.  A flag representing an incorrect answer will result, when the specimen is a weak D.




IMMUNOHEMATOLOGY, COMPREHENSIVE

Five paired, washed cells and serum (simulated recipient) and a single washed cells (simulated donor) per testing event for the following analytes:


ABO Group

Unexpected Antibody Detection and Identification

D (Rho) Typing

Compatibility Testing (Crossmatch)


ABO Group - D (Rho) Typing

      As noted above in the IMMUNOHEMATOLOGY, BASIC program.

Unexpected Antibody Detection and Identification

Compatibility Testing





IMMUNOHEMATOLOGY, COMPREHENSIVE, PLUS

Five paired, washed cells and serum (simulated recipient) and a single washed cells (simulated donor) per testing event for the following analytes:


ABO Group

Unexpected Antibody Detection and Identification

D (Rho) Typing

Compatibility Testing (Crossmatch)

RBC Antigen Typing



ABO Group - D (Rho) Typing

As noted above in the IMMUNOHEMATOLOGY, BASIC program.

Unexpected Antibody Detection and Identification and Compatibility Testing

As noted above in the IMMUNOHEMATOLOGY, COMPREHENSIVE program.

Compatibility Testing

As noted above in the IMMUNOHEMATOLOGY, COMPREHENSIVE program.

RBC Antigen Typing

Perform antigen typing on washed compatibility testing cells according to your laboratory protocol.


DIRECT ANTIGLOBULIN TEST

Two red cell suspensions, 1.0 mL.

Suspension of red blood cells for direct antiglobulin testing.

  1. Remove vials from refrigerator and allow to warm at room temperature for 15 minutes.
  2. Mix vial by rolling back and forth between the palms of the hands with occasional gentle inversion.  Do not use a mechanical mixer.
  3. Red cell suspensions are pre-washed.  Washing prior to testing is not required unless hemolysis is noted.
  4. Vials contain a 3 - 4% Red Blood Cell suspension.  If your method requires a lower percentage of Red Cells, dilute accordingly.  Typical Gel methods require a 0.8% suspension.
  5. Proceed with testing specimens as you would patient material.


D (Rh) TYPING ONLY BY SLIDE OR TUBE METHOD

Five washed red cells in suspension, 1.0 mL.

This program is for performing D (Rh) Typing only by slide or tube method. Samples are red cells in a 30-45% concentration.

Five simulated whole blood specimens per testing event

  1. Treat this sample as whole blood. The red blood cell suspension is intended to simulate whole blood with a 30-45 concentration.  For tube testing, adjust % red cells concentration per lab protocol.
  2. Code all your answers using only the numbers available on the reporting form.
  3. Laboratories NOT performing the weak D (Du) test on D (Rho) negative specimens must report code 451 for Negative, weak D (Du) not performed.
  4. Laboratories performing the weak D (Du) test on D (Rho) negative specimens must report either code 10 for negative or code 452 for weak D (Du) positive.
  5. If code 10 (negative) is reported, it is assumed the weak D test was performed and should the specimen be a weak D, a flag representing an incorrect answer will result.



FETAL RBCs

Two whole blood samples per testing event for the following:


F cell (flow cytometry)

Fetal Screen

Hemoglobin F, quantitative


These materials are made from human blood values used to determine fetal RBCs in maternal blood samples. The fetal RBCs in the product are Rho or D antigen positive and the adult RBCs are Rho or D antigen negative.  Materials can be used with both flow cytometry assays and manual stains (KB) for the detection of RBCs containing Hb-F or Rho (D antigen).

Specimen Preparation


Note to KB users: Since this material is a stabilized blood product, it may appear to stain darker or be more resistant to elution. While the adult and fetal cells are still distinguishable, using fresh eluting reagent, using room temperature fluids (25ºc), and increasing the eluting time may improve the stained appearance.


Handling and Storage

Store vials upright, tightly capped, and at 2-8°C when not in use. Avoid unnecessary cycles of warming and cooling. Protect product from freezing, from temperatures above 30°C and from prolonged time at room temperature (20-25°C).


Indications of Deterioration

The supernatant solution should be straw-colored to pink or light red. Discoloration of the supernatant fluid due to excessive hemolysis may be caused by excessive heat or freezing and may indicate product deterioration. Inability to recover expected values may also indicate product deterioration. Incomplete mixing, instrument malfunction, or defective stains are other potential causes of unacceptable results. Do not use the product if deterioration is suspected